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KMID : 0380020050200020112
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 2 p.112 ~ p.115
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Temperature Effect on the Productivity of Recombinant Protein in a Lysis and DNA packaging-deficient and Temperature-sensitive Bacteriophage λ System
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Oh Jung-Suk
Park Tae-Hyun
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Abstract
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E. coli in combination with bacteriophage was used to overcome the intrinsic plasmid instability that is frequently found in recombinant fermentation especially in long-term operation. In order to enhance the stability and productivity, the bacteriophage was used in this study. It is a mutant, which is deficient in the synthesis of protein related to DNA packaging and cell lysis. The is also a temperature-sensitive mutant. To optimize the production of recombinant protein in this temperature-sensitive system, the temperature effects on growth and cloned gene expression were investigated for stable and efficient recombinant gene expression. The induction to the lytic state was not complete at while the temperature above induced the lytic state completely. However, the productivity was decreased at by temperature inhibition. The L-free cell concentration increased with the increase of temperature until . In conclusion, has the optimal temperature at for stability and at for expression.
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KEYWORD
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Bacteriophage λ, temperature induction, productivity, plasmid instability
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